- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
This volume covers the latest methods used in clinical neurochemistry laboratories for both clinical practice and research.
Chapters in this book discuss topics such as techniques for cerebrospinal fluid (CSF) collection, pre-analytical processing, and basic CSF analysis; an examination of biomarkers including ELISA and automated immunochemical assays for amyloid and tau markers for Alzheimer’s disease; the analysis of neurofilaments by digital ELISA; and an example of successful novel immunoassay development. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Cutting-edge and thorough,
Cerebrospinal Fluid Biomarkers is a valuable resource for clinicians and researchers to use in CSF labs and CSF courses.
Table of Contents- 1. CSF Cell: Cell Count, Cytomorphology, Cytology, and Immunophenotyping
- 2. Nephelometry and Turbidimetry: Methods to Quantify Albumin and Immunoglobins Concentrations in Clinical Neurochemistry
- 3. Oligoclonal Bands: Isoelectric Focusing and Immunoblotting, and Determination of Free Light Chains in the Cerebrospinal Fluid
- 4. Abeta CSF LC-MS
- 5. Immunoassay and Mass Spectrometry Methods for Tau Protein Quantification in the Cerebrospinal Fluid
- 6. CSF RT-QuIC and the Diagnosis of Creutzfeldt-Jakob Disease
- 7. Lumbar Puncture: Consensus Guidelines
- 8. Pre-Analytical Processing and Biobanking Protocol for CSF Samples
- 9. Communicating Complex Results of Cerebrospinal Fluid Analysis
- 10. Method and Clinical Validation of Biomarkers for Neurodegenerative Diseases
- 11. Sample Preparation for Proteomic Analysis of Cerebrospinal Fluid
- 12. Brain Biomarkers: Follow Up of RNA Expression Discovery Approach - CSF Assays for Neurogranin, SNAP-25, and VILIP-1
13. Quantification of the Neurofilament Light Chain Protein by Single Molecule Array (Simoa) Array
Christian Barro, Sarah Storz, Svenya Groebke, Zuzanna Michalak, and Jens Kuhle
Subject Index List...
