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- Includes cutting-edge methods and protocols for the study of cardiac gene therapy
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
This detailed book provides methodological information on cardiac gene delivery, from classic to state-of-the-art technologies and techniques. Efficient, cardiac-specific, and safe vectors, as well as refined vector delivery methods, are key for successful cardiac gene transfer and eventually for improving patients’ outcomes. Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Gene Therapy: Methods and Protocols serves as a valuable tool for molecular biologists and physiologists in the cardiology field conducting cardiac gene transfer research, which will ultimately lead to further advancements in the vital field. Table of ContentsPart I: Overview - 1. Current Methods in Cardiac Gene Therapy: Overview
Kiyotake Ishikawa and Roger J. Hajjar
Part II: Genetic Modification - 2. Silencing Genes in the Heart
Henry Fechner, Roland Vetter, Jens Kurreck, and Wolfgang Poller - 3. Generation of Efficient miRNA Inhibitors Using Tough Decoy Constructs
Jimeen Yoo, Roger J. Hajjar, and Dongtak Jeong - 4. Efficient Genome Editing in Induced Pluripotent Stem Cells with Engineered Nucleases In Vitro
Vittavat Termglinchan, Timon Seeger, Caressa Chen, Joseph C. Wu, and Ioannis Karakikes - 5. Direct Cardiac Reprogramming as a Novel Therapeutic Strategy for Treatment of Myocardial Infarction
Hong Ma, Li Wang, Jiandong Liu, and Li Qian - 6. Production and Characterization of Vectors Based on the Cardiotropic AAV Serotype 9
Erik Kohlbrenner and Thomas Weber - 7. Cell-Based Measurement of Neutralizing Antibodies Against Adeno-Associated Virus (AAV)
Andreas Jungmann, Oliver Muller, and Kleopatra Rapti - 8. Synthesis of Modified mRNA for Myocardial Delivery
Jason Kondrat, Nishat Sultana, and Lior Zangi - 9. Exosomes-Based Gene Therapy for MicroRNA Delivery
Prabhu Mathiyalagan and Susmita Sahoo - 10. Lipidoid mRNA Nanoparticles for Myocardial Delivery in Rodents
Irene C. Turnbull, Ahmed A. Eltoukhy, Daniel G. Anderson, and Kevin D. Costa
Part IV: Gene Delivery Methods - 11. Gene Transfer in Isolated Adult Cardiomyocytes
Kjetil Hodne, David B. Lipsett, and William E. Louch - 12. Gene Transfer in Cardiomyocytes Derived from ES and iPS Cells
Francesca Stillitano, Ioannis Karakikes, and Roger J. Hajjar - 13. Gene Transfer to Rodent Hearts In Vivo
Federica del Monte, Kiyotake Ishikawa, and Roger J. Hajjar - 14. Ultrasound-Targeted Microbubble Destruction for Cardiac Gene Delivery
Shuyuan Chen and Paul A. Grayburn - 15. A Needleless Liquid Jet Injection Delivery Approach for Cardiac Gene Therapy
Anthony S. Fargnoli, Michael G. Katz, and Charles R. Bridges - 16. Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques
Shin Watanabe, Lauren Leonardson, Roger J. Hajjar, and Kiyotake Ishikawa - 17. Direct Myocardial Injection of Vectors
Guillaume Bonnet, Kiyotake Ishikawa, Roger J. Hajjar, and Yoshiaki Kawase - 18. Selective Pressure-Regulate Retroinfusion for Gene Therapy Application in Ischemic Heart Disease
Rabea Hinkel and Christian Kupatt - 19. Cardiac Gene Delivery Using Recirculating Devices
Melissa J. Byrne and David M. Kaye - 20. Molecular Cardiac Surgery with Recirculating Delivery (MCARD): Procedure and Vector Transfer
Michael G. Katz, Anthony S. Fargnoli, Andrew P. Kendle, and Charles R. Bridges
Part V: Targeting Arrhythmia - 21. Gene Delivery for the Generation of Bioartificial Pacemaker
Patrick K.W. Chan and Ronald A. Li - 22. Gene Therapy for Post-Infarction Ventricular Tachycardia
J. Kevin Donahue
Part VI: Targeting Pulmonary Hypertension - 23. MicroRNA Delivery Strategies to the Lung in a Model of Pulmonary Hypertension
Lin Deng, Andrew H. Baker, and Angela C. Bradshaw - 24. Inhaled Gene Transfer for Pulmonary Circulation
Jaume Aguero, Lahouaria Hadri, Nadjib Hammoudi, Lauren Leonardson, Roger J. Hajjar, and Kiyotake Ishikawa
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